Effect Of RhoC Gene On The Proliferation And Invasion Abilities Of Human Cholangiocarcinoma Cells

Objective. To construct sense and antisense expression vector of human RhoC and observe the effect of RhoC gene on the proliferation and invasion abilities of human cholangiocarcinoma cells.Methods. Mammalian expression vector containing RhoC cDNA or antisense RhoC cDNA was transfected into human cholangiocarcinoma cell line with Lipofectane 2000 and the positive clones were selected by Hygromycin B.RT-PCR was used to examine the expression of Hygromycin B.The cell growth curve was made to determine the inhibitory rate of cells; Boyden chamber was made to detect the invasive ability of cells; Flow cytometry was used to analyze the changes of cell cycle of cells before and after the gene transfection.Results. The results showed that all transfecters had expressed Hygromycin B. After transfection, The difference of the abilities of in vitro invasion through matrigel and proliferation in QBC939-AS group,QBC939-S group and in control group was statistically significant ( P < 0.05).Compared with the control QBC939 cells and QBC939-V cells,QBC939-AS could inhibit the growth of cholangiocarcinoma cell,and their invasiveness decreased; Flow cytometry showed higher percentage of transfected cells in G₁ phase than that of untransfected cells,but QBC939-S could increase the growth of cholangiocarcinoma cell,and their invasiveness increased, compared with those before transfected. Flow cytometry showed lower percentage of transfected cells in G1 phase than that of untransfected cells.Conclusion. Up-regulation of RhoC in cholangiocarcinoma cell line could increase the...