Antitumor Effect In SCID Mice Induced By Cancer Vaccine Prepared By Fusions Of Esophageal Carcinoma Cell Line With Dendritic Cell Derived From Cord Blood

Objective:To develop fusion vaccine of esophageal carcinoma cell and DC, and observe itsprotective and therapeutic effect against EC109 cell.Methods:1) The CD34⁺ hematopoietic stem cells were isolated from mononuclear cells prepared with Ficoll-Hypaque density-gradients centrifugation from human cord blood using CD34⁺Progenitor Cell Isolation Kit by magnetic cell sorting system (MACS);2) CD34⁺ hematopoietic stem cell were expended in RPMI 1640 medium supplemented with 10% fetal calf serum containing 300ng/ml rhGM-CSF (human Granulocyte-Macrophage Colony-Stimuting Factor) , 150 μ/ml rhTNF-α (human Tumor Neucrosis Factor α )and 200ng/ml rhSCF (human Stem Cell Factor) at 37℃, 5%CO₂ for 2 weeks as mature DCs.3) Mature DCs and then were fused with esophageal carcinoma cell line by polyethyleneglycol (PEG-3600) at the ratio of 5:1. Selecting with MACS marked with HLA-DR MicroBeads generated the EC109-DC as tumor vaccine.4) EC109, DCs and EC109-DC were washed with PBS and incubated with FA-FITC and...