Study On Construction Of Recombinant BCG-EmⅡ/3 And BCG-Em14-3-3 Vaccines Of Echinococcus Multilocularis And Theirs Immune Mechanism

ObjectiveTo construct the recombinant BCG vaccines with Echinococcus multilocularisâ…¡/3 or 14-3-3 antigen gene; To analyse the expression of E.coli-Mycobacterium shuttle plamids pBCG-Emâ…¡/3 or pBCG-Em14-3-3 by heating in BCG and E.coli Top10; To observe dynamically the immune responses of BALB/c mice induced by the recombinant BCG vaccines in intranasal inoculation groups respectively; To compare the protective immunity and immune mechanism in BALB/c mice after immunization with the recombinant BCG vaccines against subsequent challenge with protoscolexs by different inoculation routines. This could provide a kind of valuable vaccine to control alveolar echinococcosis .MethodsThe primers containing cutting sites of restriction-endonucleases were designed according to the cDNA sequence of E.multilocularisâ…¡/3 and...