Experimental Study On Apoptosis Of HL60/ADM Cells Induced By LEM-GM-CSF And Its Intracellular Concentration Of DHAQ

Objective To prepare LEM-GM-CSF and observe the cytotoxicity of HL-60/ADM cells treated with the immunoliposomes for providing the preclinical material for refractory and recrudescent AML therapy. Methods Liposome-entrapped mitoxantrone were prepared by reverse-phase evaporation (REV), high speed centrifugation was applied to separate LEM and dissociative DHAQ, colorimetry was employed to determine encapsulation efficiency, the liposomes'structure and particle size were determine by transmission electron microscopy. GM-CSF was coupled to LEM by glutaraldehyde method. UV-spectrophotometric analysis was applied to mensurate the coupled efficiency , flow cytometry analysis was applied to determine the immunoconjugate retained efficiency. The cytotoxicity of HL60/ADM cells and interdiction efficiency of GM-CSF were investigated by tetrazolium microculture (MTT) test. The intracellular concentration of DHAQ was determined by RP-HPLC. The rest data were collected from transmission electron microscopy, flow cytometric analysis. Results 1,The encapsulation efficiency of LEM was 80%; the...