Experimental Research Of Transfection Efficiency For EGFP Piasmid Transfected Into Retinoblastoma Cells By Ultrasound Microbubble Intensifier

Retinoblastoma is the commonest intraocular malignant tumor in children. Nowadays, the therapy methods include picking off eyeball, laser photocoagulation, transpupillary thermotherapy, cryotherapy, ratiotherapy et al. Gene therapy for RB is still at the starting stage. As a new gene vector, microbubble can break up and release the plasmid it carries at the appointed place by ultrasound. In the study, we detected the transfection efficiency of EGFP gene in RB cells that was mediated by ultrasound-microbubble and explored the optimum parameter of microbubble-ultrasound.The study includes three parts . The first part was about the influence of microbubble and ultrasound used to RB cells. The second part detected the best parameter of ultrasound and microbubble with which the best transfection efficiency could be reached. The third part explored the difference between the new method and the traditional one.The first part detected the influence of microbubble and ultrasound...