Investigation Of Hepatitis B Virus Genotype And The Related Mutations In Guangdong Province

Hepatitis B virus (HBV) infection is a kind of severe infectious disease that threatens the human health. The infected individuals are at high risk of developing chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. As a high endemic area and the causative agent, in China, about 300000 people will die from the HBV infection.To explore the more applicable preserving and carrying condition of the serum samples for measuring HBV-DNA level in short term at present. The serum samples of 4 inpatients with HBV infection were collected and preserved under the condition of normal temperature(22-26℃), 4℃,and -20℃, and fluorescence quantitative PCR(FQ-PCR) was performed to measure HBV-DNA level at the 5 time points, including the 1^st (Baseline), 2^nd,3^rd,5^th and 8^th day. Meanwhile, Nest-PCR was operated to amplify the HBV-DNA fragment from the 1^st and 8^th day samples preserved in normal temperature. Within 8 days, it was no significant difference that HBV-DNA level of every patient compared with their baseline under 3 temperature condition (p>0.05).And the aiming strips were all amplified clearly through the Nest-PCR. Detection and quantitation of HBV with FQ-PCR assay has been shown to be a highly sensitive, specific, and reproducible method for measuring HBV DNA levels in serum. The serum in short-term storage and transportation under normal temperature can still be detected accurately with FQ-PCR assay, and it is important to popularize the normal and reasonable prevention and cure projection for hepatitis B.