Genome Function Research Work Of Leptospira Interrogans

The genome sequencing of leptospira interrogans serovar interheamorrhagiae Chinese Lai has already completed, but this is only the first step of the research into the genome of leptospira interrogans. Leptospira interrogans serovar interheamorrhagiae Chinese Lai totally has a bigger and a smaller chromosomes, 4691184 base pair, which foreknow that it contains 4727 functional genes. Large quantities of functions of genes need us to infer and testify by the method of experimentation. It will be a challenging work to transform genome nucleic acid sequence which function is unknown into meaningful genetic functional information.Our laboratory researched into the culturing condition of leptospira interrogans serovar icterheamorrhagiae France Lai and leptospira interrogans serovar interheamorrhagiae Chinese Lai, examined the effect of glycin, Mg²⁺, DMSO, MTT, temperature on the growth of leptospira. We had mastered the culturing conditions of leptospira interrogans , and used leptospira interrogans serovar icterheamorrhagiae France Lai as the research target, groped the condition of the electric transformation of leptospira Chinese Lai, studied the resistance of leptospira interrogans serovar interheamorrhagiae Chinese Lai and also got an effective method on resistance screening, which founded a platform for the establishment of the genome-wide mutant library of leptospira interrogans serovar interheamorrhagiae Chinese Lai.It is a common and powerful method to use the transposon to mutate the gene of microbe to research the function of microbe's genome, which has been widely used in the research of genomic function of model microbe. We can find out the biological function of mutated genes by analysis of the new phenotype presented by the mutants and then locate the mutated gene by using single primer PCR. This experiment has already groped the mutation method by the Tn5 in-vitro transposition system and applied the modification of Tn5 transposition system to the establishment of leptospira interrogans serovar interheamorrhagiae Chinese Lai mutation library. We will continue to construct the transposase which promote by the promoter of...