Studies On The Chondroitinase From Aeromonas Sobria YH311

Chondroitinase has a broad future in the use of studying the glycosaminoglycans' structure, function and distribution, curing of some diseases such as intervertebral disc protrasion and preparing low molecular weight chondroitin sulfate. In this paper, the screening and determinant of chondroitinase-producing strains, the fermentation conditions and properties of the Aeromonas sobria YH311, the methods for purifying and immobilizing of chondroitinase were all studied systematically.By experiment, Strain YH311, with a high level of chondroitinase productivity, was obtained from the intestinal contents of a fish by rapid plate screening. It was identified to be Aeromonas sobria according to the biological characteristics and biochemical tests. By comparing the extracellular and intracellular chondroitinase activity of the strain disrupted by sonication, it was found that the former was much higher than the latter, which showed that YH311 secretorily expressed the chondroitinase and the chondroitinase was extracellular-enzyme.In order to increase the chondroitinase productivity, the fermentation media for chondroitinase production were inspected and optimized. The results indicated that the optical fermentation media for Aeromonas sobria YH311 to produce...