Preparation And Properties Of Sodium Alginate Bacterial Cellulose Antibacterial Composite Membrane Loaded With Nano-silver

Objective Oral and maxillofacial bone is the basis of maintaining normal oral physiological activities,maxillofacial bone loss will seriously affect the quality of life of patients,so promoting bone repair is one of the urgent tasks of oral doctors.Guided bone tissue regeneration is one of the most commonly used methods for the treatment of bone defects.In this study,by testing the tensile strength of sodium alginate bacterial cellulose composite membrane loaded with silver nanoparticles,the antibacterial activity of staphylococcus aureus and its effect on the proliferation of mouse embryonic fibroblasts,the feasibility of its use as a novel membrane to guide bone tissue regeneration was discussed.Methods First for the preparation of composite membrane material,sodium alginate(sodium salt,SA)solution and bacterial cellulose(bacterial cellulose,BC)solution by mixing proportion(the mass fraction of SA and the BC than now,they respectively,persons,10:9 and pure SA film),since the evaporation film after ultrasonic broken,then dopamine deposition on the membrane surface,and finally by dopamine in situ reduction of silver nanoparticles(Ag NPs).Secondly,the tensile strength of each membrane specimen(sample size of each group is 6)was measured by mechanical universal experimental machine.Scanning electron microscope(SEM)was used to observe the microstructure and X-ray powder diffraction(XRD)was used to analyze the composition of composite membrane specimens with the highest tensile strength.Finally,Staphylococcus aureus and mouse embryonic fibroblasts(NIH/3T3)were cultured,and the antibacterial activity of the composite membrane against Staphylococcus aureus was tested by the bacteriostatic zone method.Cell counting kit was used to detect the cell counts of the blank control group,the composite membrane group without nano-silver loading and the composite membrane group with the highest tensile strength(sample size of each group was 5)for 24 h and 48 h co-culture with mouse embryonic fibroblasts.Results The results of the universal mechanical testing machine show that the tensile strength of the composite film increases with the increase of the proportion of BC,but when the mass fraction ratio of SA to BC is 10:7,the tensile strength of the composite film reaches the maximum(223.8 ± 9.9)MPa.The silver nanoparticles were successfully infiltrated into the SA/BC composite membrane and uniformly distributed on the surface of the membrane by scanning electron microscopy.X-ray powder diffraction analysis showed that there were silver nanoparticles,sodium alginate and bacterial cellulose in the composite film.In the bacteriostatic experiment,a clear bacteriostatic circle was shown around the samples after incubation for 24 h,indicating that the composite membrane had the ability to inhibit the growth of bacteria or kill bacteria.The results of cytocompatibility test showed that there was no significant difference between the Ag-SA/BC composite membrane group and the blank group after cell culture for 24 and 48 h(P>0.05),indicating that the composite membrane had good biocompatibility to mouse embryonic fibroblasts.Conclusion In this experiment,a novel membrane material was successfully developed,and the tensile strength of the composite membrane was the highest when the AgSA/BC mass fraction ratio was 10:7.In vitro experiments showed that the composite membrane had excellent antibacterial activity and good cell compatibility against Staphylococcus aureus,and it is expected to become a good candidate for the development of a new generation of GBR membrane.