Experiment Of Hypercapnia Therapy Acute Lung Injury

Objective Detecting that whether hypercapnia would be protective against acute lung injury and its mechanisms . Method Twenty -four New Zealand white rabbits were involved in this study , Twenty -four rabbits were randomly allocated to control group (C group ). therapy group (T group) and prevention group (P group), there were eight rabbits in every group, every rabbit was injected LPS (0. 7mg/kg) intravenously to be caused acute lung injury and was ventilated in ventilator for six hours, In the course of this experiment, blood pressure and heart rate were monitored to observe the alteration of hemodynamics of rabbits; arterial blood gas was analysised to observe the alteration of partial pressure of oxygen and cardioxide partial pressure in arterial. Wet dry ratio of lung tissue of every group was detected; the cotent of malondialdehyde in lung tissue of every group was detected by colorimetric method; the contents of TNF- α in serum and BALF was detected by ELISA method; the expression of NF-κB in lung tissue of every group was detected by immunohistochemistry method and the pathology alteration of lung tissue of every group was observed in microscope at the end of the experiment .Results (1) There was no obvious difference between blood pressue and heart rate in T group and blood pressue and heart rate in C group (P>0. 05) , and There was no obvious difference between blood pressue and heart rate in P group and blood pressue and heart rate in C group (P>0.05), which suggested that hypercapnia had no obvious effect on hemodynamics of rabbits. (2) The partial pressure of oxygen and cardioxide partial pressure in T group and P group were much higher than that in C group (P<0. 01) ,and There was no obvious difference between T group and P group.