Study On Cerebral Choline Acetyl Transferase Mechanism Of Cognitive Deficits After Cerebral Concussion In Rats

Objective : To study the correlation between different injury level of cerebral concussion ( CC ) and the cognitive deficits in rats, and to explore the changes between cognitive behavior and cholinergic system of basal ganglia, prefrontal corte, middle septal area and midbrain, so as to know the neurobiologic mechanisms of cognitive behavioral deficits after cerebral concussion in adult SD rats. And provide objective neurobiologic bases for clinical diagnosis and treatment.Methods: Animal model was duplicated by using a metallic pendulum striker concussive device. The injured rats were divided into Pure cerebral concussion (PCC) group and Complicated Cerebral Concussion (CCC) group according to classification standard. The number of each group including PCC, CCC and control is 10(n=10). Beam balance (BBT)and bean walking (BWT) were measured on 1 - 5 days after injuryed to assess vestibulomotor function, coordinated walking ability, learning and memory behavior after CC. Brain Choline acetyl transferase (ChAT) expression was measured by Western blotting technique with ChAT specific antibody after PCC 1d, 2d, 4d, 8d, 16d and 24d. Additionally, 8 intact rats were used as control group.Results: Reduced latency on the BBT was observed in CCC rats (p<0.05) vs. control up to 5 days post-injury, however, PCC group did not differ from Control group. In the PCC rats, motor and equilibrium function deficits in BWT were observed in the first 2 days (p<0.05) vs. other groups, but CCC...