| Objective To investigate the correlation between different injury levelof cerebral concussion (CC) and the cognitive deficits in rats, and toexplore the changes between cognitive behavior and tryptophan hydroxylaseexpression in brainstem, so as to know about the neurobiological mechanism ofcognitive behavioral deficits after cerebral concussion in adult SD rats. Andultimately, to provide objective neurobiological fundament for clinical diagnosisand treatment.Methods A kind of metallic pendulum striker device was used to duplicateanimal models. The injured rats were divided into pure cerebral concussion(PCC) group and complicated cerebral concussion (CCC) group according toclassification standard, and a control group was deployed. There were 10animals in each group. The animals' learning and memorizing ability changeswere measured within 7 days by Morris Water Maze (MWM) test. Brain tryptophanhydroxylase(TPH) expression was detected by Western Blotting analysis.Results (1) Rats of different injury levels manifested retrogradeamnesia of distinct degree. Compared with the data got from themselves beforeconcussion, the latency of hiden platform prolonged much, while that of probetest shortened statistically. In the meanwhile, compared with the data gotfrom control group animals on each day (1-5days) after the 2 experimentalgroup animals were injuried, the hiden platform latency prolonged much,while that of probe test reduced statistically. (2) The retrograde amnesia varied with concussion of different levels. In PCC animals, it persisted from1 day to 4 days statistically, and then recovered gradually. While CCC ratsmanifested both retrograde amnesia and learning and memorizing abilitydeficit 5 days after concussion, then regained by degrees. The westernblotting analysis revealed that injuried rats' TPH expression risedstatistically from the first day till the forth day after injury comparedwith that of control group. And then, from the eighth day till the 16th day, theprotein's expression descended little by little. However, the enzyme'sexpressing action remainded higher than that of the control animals, but itwas not statistically significant.Conclusions 1. The levels of retrograde amnesia related to the injury degreeof cerebral concussion severity in rats. 2 The brainstem TPH expressionvaried before and after concussion. 3. The cognitive deficit possiblelycorrelated with the brainstem TPH expression. |
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