The Expression And Significance Of P75NTR And RhoA In Brain Tissue Of Newborn PVL Rats

Part 1: The Establishment and Assessment of the PVLModel For Immature RatsObjectiveTo establish the PVL model for the immature rats, to detect the pathological changes of brain in those with PVL and to assess their neural behavior. Methods1. A total number of 98 2-day postnatal SD rats were randomly divided into two groups: the sham surgery group and the PVL model group. Rats in the PVL model group were subjected to the ligation of right carotid artery, and then they were kept in a box with 6% oxygen and 94% nitrogen for 4 hours to establish the PVL animal model.2. Morphological Observation: Rats from both groups were executed at 12h, 24h, 48h, 72h, 7d, 14d, 28d, and HE staining method was employed on brain tissues slices of those rats. The brain pathological changes during each period of time were observed under light microscope and electronic microscope.