The Effects Of GSRd On The Expression Of CX47 And Nestin In The White Matter Of The Neonatal Rat Model With Periventricular Leukomalacia

Objective:The neonatal rat model with periventricular leukomalacia?PVL?were made in three-day-old SD rats,and detecting the expression of nestin and connexin47?CX47?in the white matter.in order to investigate the effects of GSR-d on the expression of CX47 and nestin in the white matter of the neonatal rat model with periventricular leukomalacia.Methods:The PVL model was set up in three-day-old neonatal SD rats by shearing left arteria carotis communis and then breathing 8%O2+92%N2 for one hour,which were randomly divided into PVL model group and the GSR-d trial group,and thesham operation group was made also.the pathological change of brain was investigated with microscope at the day 3,day 7 and day 14 after the operation,the expression of CX47 and nestin in the white matter of the neonatal rat model with PVL were tested by immunohistochemistry staining and image quantitative analysis in the three groups.Results:1.Hematoxylin-eosin staining of three groups of brain pathological changesThere were no changes in the sham operation group at the day 3,day 7 and day 14 after operation,the morphological structure was normal in the brain,no enlargement of the ventricles were observed.In the PVL model group and GSR-d trial group,the lesions were mainly in the white matter area,hippocampus,internal capsule,corpus callosum,and external capsule area.At day 3 after operation,in the PVL model group and the GSR-d trial group,there was a widening of the intercellular space,the tissue structure was loose,and the nerve fibers appeared screen-like necrosis and vacuolar formation,a small amount of microglial proliferation could be seen.cells edema and the loose,cells were derangement,necrosis was observed;at 7th day post operation,thenucleus could be seen to be karyopyknosis,broken and dissolved in the nucleus,white matter edema around the ventricle,loosening,mesh-like necrosis,and empty cavities were less than at day 3 after operation.The lateral ventricles were significantly enlarged at day 14 after operation,the glial cells were significantly increased,and glial scars formed,but there were still malacia and irregular structure.the degree of brain injury of the GSR-d treatment group was mild than the PVL model group.2.The expression of CX47 in periventricular white matter of three groupsAccompany with the day increasing,the expression of cx47 of the sham operating group at different time points decreased gradually.The expression of CX47 of GSR-d trial group is more than other tow groups,the second is the PVL model group,the sham operation group is the least.there is a significantly difference in three groups?P<0.05?.3.The expression of nestin in periventricular white matter of three groupsAccompany with the day increasing,the expression of nestin of the sham operating group decreased gradually,meanwhile the expression of nestin in the PVL model group and GSR-d trial group gradually increased respective.Comparing three groups;the expression of nestin in periventricular white matter of the GSR-d trial group is maximum,the second one is the PVL model group,the sham operation group minimum;there is a significantly difference in three groups at all time pionts?P<0.05?.Conclusion:1.The PVL model was set up in three-day-old newborn SD rats by shearing left arteria carotis communis and then breathing 8%O₂+92%N₂ for one hour,the the pathological presents of brain was similar with the periventricular leukomalacia,its suggested the PVL model of neonatal rat was made successful.2.Ginsenoside-Rd could relieve the pathological damage of brain in the neonatal rat model with periventricular leukomalacia,reduce the degree of enlarge of the lateral ventricle,suggesting that ginsenoside-Rd has protective effect on neonatal rats with periventricular leukomalacia model.3.Ginsenoside–Rd could enhance the expression the expression of CX47 and nestin in periventricular white matter of model rats with periventricular leukomalacia,its suggesting that ginsenoside–Rd may be involved in the regeneration of neurocytes and promoted myelin sheath format.