The Screening Of TEL-AML1 Fusion Gene And The Expression Of Multidrug Gene And Glucocorticoid Receptor Gene In Childhood Acute Lymphoblastic Leukemia

Part I The Screening of TEL-AML1 Fusion Gene in Children withAcute Lymphoblastic Leukemia Objective:To determine the incidence of TEL-AML1 fusion gene in children with acute lymphoblastic leukemia(ALL) and analyze the clinical features of TEL-AML1 positive patients. Method:Samples of bone marrow or peripheral blood of 56 newly diagnosed children with ALL were collected in our hospital from October, 2004 to January, 2006. The TEL-AML1 fusion gene was detected by the method of nested RT-PCR and further confirmed by the sequence analysis. The ALL patients were stratified into TEL-AML1-positive and negative and the clinical features were compared. Results:1. Of 56 patients, 14 (25.0%) were TEL-AML1 positive, and the sequence of nested RT-PCR products is coincided with that of the...