The Establishment Of The Novel Method To Detect Break-fusion Events Related With AML1 Gene

0bjective To establish the novel method to detect break-fusion events related with AML1 gene and search new partner genes.Method (1)The AML1/ETO fusion gene was detected in 58 acute leukaemia patients by reverse transcriptase-polymerase chain reaction (RT-PCR). (2)The mRNA expression of AML1 gene break-point region was detected in 58 acute leukaemia patients and 26 controls by real quantitative reverse transcriptase-polymerase chain reaction (RQ-RT-PCR) and using a constantly expression region of AML1 gene as internal reference.Result (1)The frequence of acute leukemia with AML1/ETO was 23.5%(16/68). None of the 26 controls was found to carry AML1/ETO fusion gene.(2) The mRNA expression of the break-point region of AML1 gene in patients with AML1/ETO fusion gene was significantly lower than that in control group(P<0.05). (3)The mRNA expression of AML1 gene break-point region decreased significantly in 22 acute leukemia patients and the frequence was 32.3%(22/68). (4)In these 22 cases, the results of the two methods of 16 cases are coincident. We screened out 6 cases which maybe carry new partner genes. These cases include 2 AML-M2 cases, 4 AML-M4 cases.Conclusion The new method can detect efficiently more significant break-fusion events associated with AML1 gene. Combined with traditional RT-PCR method, the new method can be used as a efficient method which contribute to search its new partner genes.