The Effect Of TRAIL To Ovarian Carcinoma Cell Lines SKOV-3,A2780 And PA-1

Objectives:We compared the cytotoxicity and apoptosis inducing ability of TRAIL and cisplatin in 3 ovarian carcinoma cell lines (SKOV-3, A2780 and PA-1) with different p53 status to evaluate the potential clinical usefulness of TRAIL in ovarian carcinoma chemotherapy and the importance of p53 in chemotherapy resistance. Methods:The 24hs growth inhibition of 3 ovarian carcinoma cell lines in respond to different concentration level TRAIL(25, 50, 100, 200, 400ng/ml) was determined by MTT assay. We also compare the growth inhibition curves of 3 cell lines in respond to TRAIL200ng/ml, DDP10μM and combination of DDP /TRAL respectively. The apoptosis rates of 3 cell lines treated by TRAIL200ng/ml, DDP10μM and combination of DDP /TRAL respectively for 24hs was measured by flow cytometry. Comparisons among the values were performed using ANOVA. A value of P <0.05 was considered statistically significant. Results:We observed the 3 cell lines treated by TRAIL for 24h at the...