Urinary Bladder Epithelial Cell-pathogen Interaction: Studies On Antibacterial Activity Of Human Urinary Bladder Epithelial Cells

The host defense mechanism against infection is based on two distinct recognition system: nonclonal recognition of innate immunity and clonal recognition of accuquired immunity. The innate nonclonal immunity system is more important so many research focus on it. TOLL-like receptor is one of the important members of innate immunity, it is a Patter recognition receptor PRR that recognize the conservative motif — Pathogen associated molecular pattens. Toll-like receptor induces the innate immunity that will activate accuquired immunity.Uropathogenic Escherichia coli (UPEC) are the major causative agent of urinary tract infections (UTIs). UTIs are considered acute, self-limiting infections despite the revalence of recurrent symptoms two or more times within months of a primary infection. Several in vitro and in vivo models of infection have provided insights into the progression of UPEC-induced UTI. We recently described the antibacterial activity of T24 cell line against intracellular Escherichia coli through 48hr.The clearance of different types of Escherichia coli in the T24 cell is found in our experiment. Control and activated confluent T24 monolayers in 48-well microtitre plates were incubated...