The Effect Of Lactobacillus On The Level Of P-p38MAPK And Apoptosis Index In SGC-7901 Cells Treated By Hp Or Hp-LPS

Objectives:To investigate the effect of lactobacillus bulgaricus(LBG) on the level of Phosphorylated p38 mitogen-activated protein kinase(P-p38MAPK) and apoptosis index in SGC-7901 cells treated by helicobacter pylori Sydney strain l(HpSSl) or lipopolysaccharide of helicobacter pylori Sydney strain l(HpSSl-LPS).Materials and Methods: The cells are divided into thirteen groups:NC group(control group),Hp group(treated with HpSS1),LPS1,LPS2, LPS3(treated with 2.5 , 25 , 250EU/ml HpSSl-LPS respectively), LBG-Hp(treated with LBG for 1h followed by HpSS1 treatment). LBG-LPSK LBG-LPS2, LBG-LPS3(treated with LBG for 1h followed by 2.5, 25, 250EU/ml HpSS1-LPS treatment respectively), SB-Hp(treated with SB203580 for 1h followed by HpSS1 treatment), SB-LPSK SB-LPS2, SB-LPS3(treated with SB203580 for 1h followed by 2.5, 25,250 EU/ml HpSS1-LPS treatment respectively).Measure the level of P-p38MAPK with immunocytochemistry ,the cell viability with MTT and the apoptosis index...