Association Of The HLA-DQA1 Alleles With Lupus Nephritis In Guangxi Han Nationality Patients

Objective: To explore the association of HLA-DQA1 alleles with Systemic lupus erythematosus of Han nationality in Guangxi.Methods:Polymerase chain reaction-sequence specific primers technique(PCR-SSP)was used to comparatively study the allelic genes in HLA-DQA1 locus in 64 patients with SLE and 60 selected unrelated healthy controls of Han subjects.Results: Twelve pairs primers amplified out 10 isoforms within HLA-DQA1 region:HLA-DQA1*0101,0102,0103,0104,0201,0301,0302,0401,0501,0601。The allelic frequencies of HLA-DQA1*0101,0102 was significantly increased in SLE patients than those of the controls(χ2=8.627,P=0.003 ,RR=3.421;χ2=8.965,P=0.003 ,RR=3.314); The allelic frequencies of HLA-DQA1*0102 was significantly increased in LN patients than those of the non-LN SLE patients(χ2 =6.418 , P=0.011, RR=4.688),but the allelic frequencies of HLA-DQA1*0501 was significantly decreased in LN patients than those of the non-LN SLE patients(χ2 =6.130 , P=0.013,RR=0.172);We have no found the association of HLA-DQA1 alleles with the renal pathologic of LN.Conclusion:The HLA-DQA1*0101,0102 allele may be susceptible gene for SLE in Guangxi Han nationality.The HLA-DQA1*0101 may be susceptible gene for LN in Guangxi Han Nationality. The HLA-DQA1*0501 may be protecting gene for LN in Guangxi Han Nationality. We have no found the association of HLA-DQA1 alleles with the pathologic of LN.