Study Of The Factors Relating With The Reconstruction Of Excitatory Neural Network In Hippocampus After Epilepsy

Partâ… :Study of the Changes of EphA5 Receptor and EphrinA3 Ligand in Hippocampus of Rat Following Kainic Acid Induced Epileptic SeizureObjective: To study the gene expression change of axon guidance factor EphA5 receptor and its ephrinA3 ligand, and explore the effect and relationship with excitatory neural network in hippocampus after mesial temporal lobe epilepsy (MTLE).Methods: Establishing the MTLE model of adult rat following kainic acid induced epileptic seizure by micro-injection kainic acid in lateral cerebral ventricle. Setting up NS control and experimental groups .Observing dynamic change of the expression of EphA5/ephrinA3 mRNA in hippocampus of rat brains after epileptic seizure between 1d,1w,2w,3w,4w.by using in-situ hybridization methods, and quantitatively analyzing the dynamic movement by computer imaging analytical technique.Results: At granular cell layer of dentate gyrus and pyramidal layer of CA3 region in hippocampus, the expression of EphA5/ephrinA3 mRNA began increasing at 1w, 2w achieving the peak, 4w getting close to control group level.Conclusion: The increasing expression of EphA5/ephrinA3 mRNA in hippocampal neuron after KA induced epileptic seizure could regulate mossy fiber sprout and synaptic reconstruction in hippocampus of MTLE rats, which these were possible mechanism of the formation of stable abnormal excitatory neural network after epilepsy.