Effects Of Triamcinolone Acetonide On Proliferation And Apoptosis Of Hypertrophic Scar Fibroblasts In Vitro

Objective: To study the effcets of diffent concentration and action time of Triamcinolone Acetonide on the proliferation and apoptosis of hypertrophic scar fibroblasts in vitro; to investigate the mechanism of Triamcinolone Acetonide in treatment of hypertropic scar. And to find out the optimal concentration of Triamcinolone Acetonide for clinical treatment of hypertropic scar.Methods:1. Collect tissue of hypertrophic scars from the hospital.2. Culture and purify the HFB in vitro in the tissue cultured system.3. The cultured HFB were identified by the morphological and immune-histochemistrical methods.4. Different concentration of Triamcinolone Acetonide were administered to the cultured HFB for 24hours, 48hours, and 72hours.Set the non- Triamcinolone Acetonide as negative contrast.5. The proliferative activity of HFB were detected by MTT method.6. The electric-microscope and the Tunel method were used to detect the apoptosis of HFB.7. Collect and analyze data.Result:1. The cultured cell were proved of HFB by the morphological evidence and the immune-histochemistrical evidence.2. The MTT test showed that : The inhibiting rate of HFB was increasedobviously with the concentration of Triamcinolone Acetonide(P<0.01). The inhibiting rate was in proportion to the administered time and theconcentration of Triamcinolone Acetonide. And the IC50 in 24h,48h,72h are0.42g/L,0.21g/L and 0.10g/L.The IC95 in 24h,48h,72h are...