Effects Of Ketamine On Synaptic Transmission,NMDA-NO-cGMP Passageway And Neurotransmitters In Sympathetic Ganglia Of Rats

Objective Observe the effects of Ketamine (Abbreviate: Ket) on the bioele- ctriccalphenomenon of CG cell in the control rats and on NMDA-NO-cGMP passageway and neurotransmitters of the sympathetic ganglia neurons in the incision pain model of rats. Methods (1) Intracellular recording techniques was used to investigate the effects of different concentration (3,1 and 0.3mmol/L) Ket on AP and RMP of CG cell in the control rats. (2) To observe the effects of small dosage (10 and 20mg/kg) Ket on NMDA-NO-cGMP passageway and neurotransmitters of the sympathetic ganglia in the incision pain model of rats according to the Brennan's methods. NO kit (Nitrate reductase method) was used to measure NO content. NOS kit was used to measure NOS activities. Coomassie kit was used to measure tissue protein concentration. RIA was used to measure cGMP content. RT-PCR method was used to measure NMDAR1 gene expression. HPLC-FLU was used to measure NE,DA and 5-HIAA content. Results (1) The inhibition of 3,1 and 0.3mmol/L Ket on AP was reversible and concentration dependent and Ket could alter RMP into hyperpolarization. (2) Cumulative pain scores equation in 10 and 20mg/kg Ket groups were higher than model, however, cold stimulative scores were lower than model. When compared with normal saline group, there were statistical difference. (3) When compared to model group, 10 and 20mg/kg Ket could decrease NO and cGMP contents, attenuate NOS activity, inhibit NMDAR1 gene expression and increase the contents of NE,DA and 5-HIAA in the sympathetic ganglia neurons. When compared with normal saline group, there were statistical difference. Conclusion (1) The Ket effect on NMDAR in the sympathetic ganglia neurons was voltage-depedent. (2) After Ket inhibited NMDAR, it reduced the...