Influence Of Nimesulide On The Release Of PGE₂ And The Expression Of Survivin In Human Tongue Squamous Cell Carcinoma Tca8113 Cell Lines

Objective: To observed the change of PGE2 and Survivin protein in Human tongue squamous cell carcinoma Tca8113 cell lines by inhibiting activated COX-2 for investigating potential mechanism of COX-2 and the selective COX-2 inhibitor nimesulide affect biological behavior of human squamous cell carcinoma.Methods: Tca8113 cell were cultured in vitro,and exposed to a range of concentrations of nimesulide NIM(25,50,100,200μmol/L)for 24h,48h,72h and the PGE2 level in the cells cultured Supernatant was quantitated by the radioimmunoassay,and Expression Of Survivin in Tca8113 cell Were measured by the SP immunohistochemistry staining simultaneously.Results: Tca8113 cell were treated with NIM(25,50,100,200μmol/L)for 24h,48h,72h and the PGE2 production was significantly decreased in the experimental group than that in the control group(43.21±1.14 pg/ml)(P<0.05).Expression of Survivin in the experimental group was decreased than that in the control group(0.39±0.03)(OD) (P<0.05).By analysis of variance, the PGE2 production and Expression of Survivin were reduced in a concentration and time dependent manner. The correlation coefficient of PGE2 and Survivin was 0.95, P<0.01, so the variational tendency of PGE2 and Survivin was coincident.Conclusions:1) The PGE2 level in the cultured Supernatant decreased by NIM in a concentration and time dependent manner.2) Expression of Survivin significantly reduced in the same manner.3) The variational tendency of PGE2 and Survivin was highly coincident.4) NIM can inhibit Cox-2 activity and down-regulate PGE2 and Survivin Expression, which may be involved in cancer inhibition mechanisms of Cox-2inhibitor.