Role Of Endothelin Biological Effect Antagonists In Renal Ischemia-reperfusion Injury And Its Underlying Mechanisms In Rats

Objective: Endothelin (ET) is a polypeptide produced from the endothelial cells of blood vessel. It is the most potent vasoconstrictors known so far. ET not only takes part in the regulation of renal function under normal condition, but also plays an important role in the renal disease. Antagonizing the biological action of ET might be beneficial effects to prevention and cure renal disease. However, the roles of ET biological effect antagonists in renal ischemia-reperfusion injury and their underlying mechanisms remain unknow. The present study was undertaken to observe the role of ET and ET biological effect antagonists:α-lipoic acid, Nifedipine and L-argenine in RIRI and their underlying mechanisms by using the physiological, biochemical, immunochemical methods and Flow cytometer techniques. The results were as follows:1 Effect of alpha-lipoic acid on renal ischemia-reperfusion injury in ratsMethods: The model of RIRI was induced by bilateral clamping the renal artery and vein for 45 minutes followed by reperfusion. After two days breeding in 18-22℃circumstance, Forty-two male Sprague-Dawley rats weighing 220-250g were...