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An Experimental Study On The Anti-tumor Effect And Mechanism Of Traditional Chinese Medicine Oldenlandia Diffusa Willd

Posted on:2006-05-11Degree:MasterType:Thesis
Country:ChinaCandidate:J H TangFull Text:PDF
GTID:2144360185953028Subject:Pharmacology
Abstract/Summary:PDF Full Text Request
Objective: To investigate the anti-tumor effect and mechanism of traditional Chinese medicine oldenlandia diffusa willd.Methods: The model bearing tumor mouse were constructed by implanting subcutaneously S180 and H22 tumor cells, then were treated with oldenlandia diffusa willd extracts (ODE and ODW) to observe its anti-tumor activity in vivo; Immuno- modulating activity was observed by the spleen index and thymus index measured; The change of S180 and H22 KM mice tumor tissue and cell morphology were observed by pathological HE staining technique; The suppressive effect of oldenlandia diffusa willd extractive of the different organic solvent on the proliferation of K562 tumor cells were assayed in vitro using cell culture technique and MTT colorimetric method; Oldenlandia diffusa willd ethanol-extractive (ODE) was purified and isolated using chromatogram technique, The structure of the simple compound from ODE was identified by spectrum technique, The anti-tumor effect of the simple compound-Scopoletin on the proliferation of K562 tumor cells was measured in vitro using cell culture technique and MTT...
Keywords/Search Tags:Oldenlandia diffusa willd extracts (ODW, ODE), anti-tumor activity, anti-tumor mechanism, immuno-modulating activity, apoptosis, simple compound
PDF Full Text Request
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