An Experimental Study On The Anti-tumor Effect And Mechanism Of Traditional Chinese Medicine Oldenlandia Diffusa Willd

Objective: To investigate the anti-tumor effect and mechanism of traditional Chinese medicine oldenlandia diffusa willd.Methods: The model bearing tumor mouse were constructed by implanting subcutaneously S₁₈₀ and H₂₂ tumor cells, then were treated with oldenlandia diffusa willd extracts (ODE and ODW) to observe its anti-tumor activity in vivo; Immuno- modulating activity was observed by the spleen index and thymus index measured; The change of S₁₈₀ and H₂₂ KM mice tumor tissue and cell morphology were observed by pathological HE staining technique; The suppressive effect of oldenlandia diffusa willd extractive of the different organic solvent on the proliferation of K₅₆₂ tumor cells were assayed in vitro using cell culture technique and MTT colorimetric method; Oldenlandia diffusa willd ethanol-extractive (ODE) was purified and isolated using chromatogram technique, The structure of the simple compound from ODE was identified by spectrum technique, The anti-tumor effect of the simple compound-Scopoletin on the proliferation of K₅₆₂ tumor cells was measured in vitro using cell culture technique and MTT...