Rabies Virus Liposome Vaccine Research

Objective: Rabies is the common disease of human and animalwhich is arise from rabies virus. Human and all endotherms arevulnerable. Rabies is deadly disease, once it come on , no method can betaken. Injecting rabies vaccine is the only remedy. At present, the rabiesvaccine is vero cell purified vaccine that plus aluminium. It has poorpotency. The sufferer must be immuned for five times in a month. Thethesis is about rabies liposome vaccine, which can enhance the potencyand decrease immunity times. Methods: the material is about PC,CHOL and SA, the freeze-dried protectant is fucose. Rabies liposomevaccine is prepared by REV-DRV method. Its modality is observed withelectron microscope. Its particle diameter is measured. At the same time,we mensurated enveloped efficiency and release rate outside. Abouttoxicological experiments , hypersusceptibility experients , security andlocal stimulating have been done. At the end, the thesis give theevaluation about its immunity. Including potency agaist rabies(NIH), viralneutralizing antibody(RFFIT), lymphopoiesis experimentation(in vitro),NK cell activity. Results: the rabies liposome vaccine modality thatprepared with REV-DRV, take on rotundity or ellipse. Its particle diameterdistribute uniformity. Average particle diameter is about 12.25μm;Eveloped efficency exceed 65%, this sample is innocuity and noinstimulation. Both hypersusceptibility and security experimentationmeasure up. The rabies vaccine with liposome as vector can enhancepotency 3 multiple compared with the vaccine plused aluminium. Aboutthe liposome vaccine,its viral neutralizing antibody has been enhanced,there are remarkable difference between two groups. The sampleenhanced the cellular immunity. Lymphopoiesis index ,NK cellactivity ,IL-2 and IFN activity is higher than the counter. there areremarkable difference between two groups. Conclusion: The rabiesliposome vaccine which prepared by REV-DRV enhance cellular andhumoral immunity, and enhance immunity potency against rabies. Theinjecting times may be decreased in the immunity process against rabies.