Establishment Of The Method Of SNP Genetic Monitoring And The Molecular Biological Study Of Biochemical Markers Of Mice

Genetic monitoring technique is one of important means to the genetic quality control, but the existing monitoring methods can't meet the need of development of science. During the research of human genome for the past few years, SNP has been the focus of much attention in genetics, but the study of SNP of mice, especially the study of SNP applicated in genetic monitoring to inbred mice, is very scarce. So to establish the method of SNP genetic monitoring and study the relationship of biochemical markers and SNP, can consummate the genetic monitoring technique to laboratory animals and improve the level of monitoring of our country.The paper can be divided in two parts: (1) Establishment of the method of SNP genetic monitoring of mice Five abroad inbred mouse strains were detected by 16 SNP loci, using single-tube bi-directional allele specific amplification (SB-ASA); the double-blind experiment and sequencing was designed to validate the reliability of the method. 16 SNP loci of 5 abroad inbred mouse strains were successfully genotyped by the method of SB-ASA, and the results were completely consistent with sequencing. The five inbred mouse strains were accurately distinguished by just 3 SNP loci based on the double-blind experiment. Besides, 4 domestic inbred mouse strains were detected, and the alleles of 16 SNP loci were identified. The allele maps of 16 SNP loci for 9 inbred mouse strains were drawed to provide effective experiment data for the genetic identification of mouse strains and distinguishing among mouse strains, so that the more accurate genetic background information was offered for genetic monitoring to the inbred mouse.(2) The molecular biological study of biochemical markers To study the mechanisms and correlation with SNP of polymorphism of biochemical markers at the molecular biological level, Car2, Gpi1 and Hbb were chosen to be analysed on 4 different aspects of biochemical electrophoresis, DNA, RNA and translational protein. During the study, the possible basis for the two electrophretic types, Car2~a and Car2~b, of inbred mouse strains was detected as a Gin/His interchange at position 38. The difference at amino acid residue 247, Phe/Leu, might account for the two electrophretic types, Gpi1~a and Gpi1~b. The basis for the two electrophretic types, Hbb~d and Hbb~s, of inbred mouse...