Studies On Extraction, Purification And Function Of The Active Components In Atractylodes Macrocephala Koidz.

The extraction, separation, purification, structural features and function of the active componentsflavones, essential oil and polysaccharides seperated from Atractylodes Macrocephala Koidz(AMK) were investigated. Atractylodes Macrocephala Koidz is a feverfew plant which has been publicized as one of edible Chinese herbs by National Sanitation Department.With the uniformity design of U₅(5³), the optimal conditions of extraction of flavones were obtained as follow: adding 18.24 times of 95% ethanol as much as Atractylodes Macrocephala Koidz Rhizoma's weight in 80℃ for 120 min by 1 time extraction. With the uniformity design of U₅(5³), the optimal conditions of extraction of essential oil were obtained as follow: adding 14 times of petroleum ether as much as Atractylodes Macrocephala Koidz Rhizoma's weight in 85℃ for 150 min by 1 time extraction. With the orthogonal design of L₉(3⁴), the optimal conditions of extraction of water-soluble polysaccharides were obtained as follow: adding 21 times of water as much as the weight of AMD powder. Coarse water-soluble Atractylodes Macrocephala Koidz Rhizoma polysaccharides (CAMP) were purified by denionzing, decolor, deproteinze, dialysis and deposition in order to get Refined Atractylodes Macrocephala Koidz Rhizoma polysaccharides (RAMP). AMP-1 and AMP-2 were separated by DEAE-cellulose column from RAMP, and then they were purified by Sephedax G200 gel column. The results showed that AMP-1 was a neutral and pure polysaccharide, AMP-2 was a acidic polysaccharide which consists of two fractions, AMP-2a and AMP-2b.The molecular weight of AMP-1, AMP-2a, AMP-2b was about 6732.87, 17450.18, 5977.23 and 5896.43, 18239.82, 6509.44 which were obtained by gel-filtration method and HPLC method. GC analysis showed that fructose, arabinose, glucose are the main components in RAMP and AMP-1.All the active components in AMD have the function of anti-oxidation. The flavones is a good radical scavenger, when at a low concentration ( 0.434mg/mL), the scavenge rate goes up to 87.84%. So does the essential oil, whose scavenge rate is 40.97% at the concentration 0.137mg/mL. AMP can inhibit the activity of a-amylase to some extent.This research provides scientific reference for the further study and development of AMK as a natural plant resources in China. Besides, it enriches the theory of food chemistry and food nutrition.