1.Association Study Between VDR And HLA-DQA1 Gene Polymorphisms And The Outcomes Of Hepatitis B Virus Infection In Northern Chinese Han Population 2.Genetic Polymorphisms Of RNase L And HLA-DQA1 Genes And Response To Interferon α Therapy In Chronic Hepat

Individuals with hepatitis B virus (HBV) infection could become spontaneously HBV resolved, no asymptomatic HBV carrying, and chronic hepatitis B. Chronic infection with HBV is a frequent cause of cirrhosis, hepatocellular carcinoma, and liver-related mortality worldwide. Besides viral and environmental factors, these different outcomes are due to the variety of host immune response. Recent studies suggested that 1,25-dihydroxycholecalciferol [1,25(OH)₂D₃] involved in the immune regulation after combining with vitamin D receptor (VDR), which might be ralated with the immune response after HBV infection. Another studies showed that human leukocyte antigen (HLA) might play an important role in the outcomes of HBV infection.This study adopted candidate genes strategy and case-control design. In northern Chinese Han population, chronic hepatitis B patients, asymptomatic HBV carriers and spontaneously recovered HBV subjects were recruited. Genomic DNA was extracted from peripheral blood samples. Genotypes were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-sequence specific primer (PCR-SSP) techniques. The frequencies of the polymorphisms of Fok I locus (exon 2) and Taq I locus (exon 9) of VDR gene and HLA-DQAl gene (exon 2) in three groups were compared using x²test for P value, odds ratio (OR) and 95% confidence interval (CI) to determine the influence of host genetic factors on HBV infection outcomes. The results could provide theoretical basis for demonstrating genetic susceptibility to hepatitis B and predicting the outcomes of HBV infection to some degree. The results are as follows: 1. Association study between VDR gene polymorphisms and the outcomes of hepatitisB virus infection 1) The univariate analysis showed a significant difference in Fok I polymorphisms...