Study On Association Between Vitamin D Receptor Gene Polymorphisms And The Chronic Hepatitis B

Objection: To investigate whether the vitamin D receptor gene polymorphisms are associated with the chronic hepatitis B.Method: 53 patients and 56 healthy peoples were studied. Polymerase chaim reaction-restriction fragment length polymorphism (PCR-RFLP ) was used to detect the polymorphisms of FokI locus in exon 2 and BsmI locus in intron 8 of VDR gene. The comparision of frequence of genotype between the patients and controls were analyzed by Chi-square test.Results: (1) In 53 patents, the genotype frequency of BB,Bb and bb was 9.4%, 20.8% and 69.8%, The BB genotype frequency in patients group was higher than that in normal control group, and there was a significant correlation P < 0.05 ) .(2) The frequence of B alleles in chronic hepatitis B patients group was 19.8%, higher than that 8.9% in control group (P < 0.05 ) . (3) The frequence of genotype of FF,Ff and ff was 32.1%, 52.8% and 15.1% in patients, and there was no significant correlation between patients and normal control group. (4) At the sametime, the allele frequency of F in the chronic hepatitis B group was higher than that in the normal control group(58.8%vs 50.9%), but there no significant corretation between the two groups. Conclusion: VDR gene polymorphisms may be related with chronic HBV infection, especially the allele B of BsmI locus may cause chronic HBV infection.