Proteome Study Of Colorectal Cancer Genesis And Hepatic Metastasis

Colorectal cancer is one of the popular malignant tumors in our country. A large number of statistical data indicates that the rates of incidence and mortality of the disease in our country have been going up in recent years. Hepatic metastasis caused severe and fatal effect on patients that underwent radical resecsion for large intestine primary cancer. Early forecast and diagnosis of liver metastasis is the key to improving survival rate for large intestine cancer patients. Now proteome study methods and techniques, especially two-dimensional electrophoresis (2-DE), provide useful and important support for the research of hepatic metastasis of large intetine cancer.[Objective] To study differential proteins and their biological functions associated with colorectal cancer genesis and hepatic metastasis by proteomics and molecular biology techniques.[Methods] Isoelectric focusing/SDS acrylamide gel two-dimensional electrophoresis was used to analyse expression of differential proteins among normal colorectal mucosa, primary cancer lesion and hepatic metastasis. Liquid chrom atography /mass spectrometry(LC-MS/MS) was used to identify the differential proteins. Transfection experiment of rectum cancer HR-8348 cells was performed with the differential protein cDNA, and the changes of cell biological behavior was observed.[Results] Significant differences of protein expression were found on two-dimensional electrophoresis. Thirteen differential protein spots were analysed and identified. Human carbonic anhydrase II was detected in normal colorectal mucosa , but lost in primary cancer lesion and hepatic metastasis. phosphoglycerate kinase 1, fumarate hydratase and aldolase A were expressed in primary cancer. Expression of homo sapiens arginase and homo sapiens glutathione S-transferase A3 was found in hepatic metastasis, but not in primary cancer lesion. After transfection with human...