The Influence Of Hypertonic Saline Preconditioning On Renal Ischemia-reperfusion Injury In Rats

Background and Objective: Kidney belongs to hypertransfusion organ, and is very sensitive to ischemia or hypoxia. Renal ischemia-reperfusion injury has been an important factor leading to acute renal failure(ARF) after trauma hemorrhagic shock resuscitation, aortic aneurysm operation, and some renal operations such as renal tumorectomy and kidney transplant. However, how to prevent and treat renal ischemia-reperfusion injury is still an interesting topic. Hypertonic saline(HTS), as a new kind of resuscitation fluid, has manifested in protecting important organs such as heart, lung, brain, etc and decreasing the occurrences of acute respiratory distress syndrome (ARDS) and multiple organ dysfunction syndrome (MODS), and improving survival rate of animals when small dose of hypertonic saline is quickly administrated at early stage of traumatic-hemorrhagic shock. But whether hypertonic saline preconditioning has advantage to the secondary ischemia-reperfusion injury of regional organ is scarcely reported. Because renal ischemia-reperfusion injury is common and still difficult to handle clinically, the influence of hyertonic saline on renal ischemia-reperfusion injury with clinic routine dose and concentration was studied on classic model of renal ischemia-reperfusion in rats in order to explore its possible mechanisms of hypertonic saline preconditioning, and provide reasonable and experimental evidences for new use of hypertonic saline in prevention and treatment of renal ischemia-reperfusion injury clinically.Methods:1. The model of renal ischemia-reperfusion in rat was set up with removal of right kidney, and clamping left renal pedicel inducing left kidney ischemia and following reperfusion in situ.2. Fifty-six healthy male Sprague-Dawley rats were divided randomly into sham-operated group, normal sodium pretreating renal ischemia -reperfusion injury group and hypertonic saline preconditioning renal ischemia-reperfusion injury group. 7.5% hypertonic saline was injected by tail vein in 2 minutes at a dose of 4mg/kg, which is...