The Reaction Of T Lymphocyte Subsets Of Ginkgo Biloba Extract In Balb/c Mice With Acute Cerebral Ischemic Stroke

Objective: Ginkgo biloba extract (GBE) have neuroprotective properties. PAF antagonism , free-radical and NO scavenging, interaction with neurotransmitters are possible mechanisms of action. Stroke is associated with changes in immune responses. Cerebral ischemia triggers acute inflammation, which exacerbates primary brain damage. Activation of the innate immune system is an important component of this inflammatory response. Lymphocytes enter the nervous system not only in autoimmune diseases, but also in response to primarily 'non-immune' neuronal damage such as stroke. T-cell activation provides a unique opportunity for early identification of stroke risk and targets for future therapy. We adopted focal acute ischemic stroke model by photothrombosis in Balb/c mice to evaluate lymphocyte proliferation and the distribution of T lymphocyte subsets, combining with TTC and Nissl staining after GBE pretreatment. We hope that it can help to identify acute ischemic stroke risk and to find new targets for future therapy.Methods: 120 adult Balb/c mice were randomized to divide into four groups:(1) Simple injection Rose Begnal group, which is as control group. (2) Ischemia group. (3) GBE pretreatment group. (4) Dexamethasone treatment group, as a positive control. GBE pretreated with intraperitoneally doses of 100mg/kg/day. On the 7th day,mice were anaesthetized and photothrombotic focal cerebral ischemia were induced. Injection of the photosensitive dye Rose bengal via vena caudalis,subsequent focal illumination of the brain with a cold light source through the intact skull led to focal cortical infarcts. Animals were sacrificed at...