Experimental Study Of Cardiac Tissue Engineering

Background Heart disease is the number one cause of death in industrialized nations. Myocardial infarction and heart failure resemble the most prevalent pathologies. The human heart cannot regenerate significantly because adult cardiac myocytes are terminally differentiated and cannot replicate after injury. In end stage heart failure heart transplantation remains the last treatment option with good long-term results. Unfortunately, heart transplantation is limited due to an inadequate supply with donor organs. Despite elaboration of pharmacological and surgical treatments, the number of patients with heart failure is increasing. Thus, there is an obvious need to improve traditional treatment and develop new strategies to cope with heart failure in the future.Objective The present study was first conducted to develop the strategy for engineering cardiac tissue using neonatal rat cardiomyocytes which are comparatively more stable in phenotype during in vitro culture. Based on this strategy, cardiomyocytes derived from embryonic stem (ES) cells were used to engineer cardiac tissue with a long-term goal for repair or replacement of infracted or diseased myocardium.Methods (1) Cardiac heart tissue engineering using neonatal rat cardiomyocytes. Liquid collagen type I, Matrigel and 2 X DMEM were mixed with neonatal rat cardiac myocytes isolated using 0.25% trypsin including 0.04%...