| Objective : To explore the possibility of culturing Adipose tissue derived stromalcells (ADSCs) in large quantity by microcarrier suspension system and the multidifferentiationability of the cultured cells.Methods: Adult human ADSCs cultured on microcarriers in spinner flasks were compared with those of statically cultured in 12 well plates. For the adherent growth of ADSCs, Cytodex 3, one kind of microcarries,was use d at the concentration of 5 g /L. The cells were seeded in adensity of 1×10~8 /L in both cultural systems.Results:After 9 days of cultivation, the results showed that amaximum viable cell density ( 1.5×10~9cells /L) was reached in spinner cultured system , where as the cell density increased to amaximum of 3.4×10~8 cells /L on the 7 th day in static culture system. After spinner culture for 11 days, the ADSCs still maintained the characteristics of stem cells.CONCLUS ION: Microcarrier culture system is aneffective way to expand ADSC s as the seeding cells in tissue sengineering.
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