Preparing Antisense-oligonucleotides Anionic Liposome And Investigate Its Effect On The Resistance Of MRSA

AIM: Bacteria resistance is a common problem in clinical therapy, which caused big difficulty in controlling infection. Methicillin-resistant staphylococcus aureus(MRSA) is a common resistant bacteria, once the bacteria is resistant to methicillin, it will be resistant to all P-lactams antibiotics. So it is important to make MRSA sensitive to P-lactams antibiotics again. It is believed that resistance owing to synthesis new PBP2a protein and P-lactamase, they are encoded by mecA and blaZ. MecRl/MecI regulates mecA, while BlaR1/BlaI regulates blaZ. Antisense drugs can combine to targeted gene selectively and block the expression of gene, then have effect on therapy. So we try to choose antisense drugs to selectively...