Expression, Purification Of Plasmodium Berghei Homologue Of Macrophage Migration Inhibitory Factor, And Detection Of Its Expression In Blood Stage Parasites

AIM: Malaria is still one of the most deadly infectious diseases. Malaria eradication has been hampered by lack of knowledge about Plasmodium's biological characteristics and host-parasite relationship. Many new genes have been found thanks to the sequencing of the entire genome of Plasmodium. Study on these new genes will let us know more about Plasmodium, and accelerate the development of vaccines and drugs. Macrophage migration inhibitory factor (MIF) is one of the first cytokines discovered and is one of the key regulators of immune system in mammals. Homologues of MIF have been found in genome of Plasmodium. Study on Plasmodium homologues of MIF may help us know more about Plasmodium's biological characteristics and host-parasite relationship. In order to study biologic functions of Plasmodium's MIF homologues, we expressed and purified P.berghei homologue of macrophage migration inhibitory factor (PbMIF). Mouse antiserum against PbMIF had also been prepared, and expression of PbMIF in blood stage P.berghei had been...