The Changes Of Cellular Immune Function And The Correlation Of TGF-β1 Levels In Serum On The Patients Of Hepatocellular Carcinoma

Hepatocellular carcinoma with the pathological characteristics of high aggression,early metastasis,tendancy to recurrence and poor prognosis is one of the most common malignant tumors.The metastasis and recurrence are a complicated process with multi-steps and also the major factors to influence the curative effect. Nowaday the researches show that there is a close relationship between the invasion and metastasis of malignant tumor with the transforming growth factor-betal( TGF-β₁) level. Objective:By the means of detecting the changes of cellular immune function of hepatocellular carcinoma and the effection of TGF-β₁ levels in serum on proliferation and cellular immune function of the peripheral blood mononuclear cell(PBMC), we can explore the function of TGF-β₁ and CD4⁺CD25⁺T subsets proportion immuno-activity in the progression of hepatocellular carcinoma (HCC) and try to investigate the mechanism of immuno- suppression in HCC and provide evidence for effective intervention in cellular immune function. Methods:The tumor tissue was collected from forty five patients with HCC; The peripheral blood was collected from forty five patients with different stage of HCC and twenty healthy people. The proportion of CD4⁺, CD8⁺, CD4⁺CD25, CD4⁺CD25⁺CTLA4⁺ T subsects was evaluated and analyzed by triad colour immunofluorescence labelling on flow cytometry and the level of TGF-β₁ was...