Cloning Of Combiningantigen Of HBsAg And Human Parvovirus B19 VP2 And Its Expression In E.coli.

Background and Objective:Human parvovirus B19(B19), the only parvovirus known to be pathogenic in human, belongs to the genus Parvovirus in the family Parvoviridae. It is the smallest animal DNA virus, and transmitted mainly by respiratory tract and blood, which can cause local epidemic in certain communities. B19 was reported that it is one of the important pathogens causing acute aplasia anemia, non-immunity hydrops, acute trombocytopenic purpura and other diseases since B19 infection had been confirmed its existence in China in 1990. And now it is certified that B19 is associated with a variety of clinical manifestations in adults and children. Women and children in domestic are sensitive to B19 infection because of the low level of the protective antibodies in their sera. B19 infection in those with low immunity function or immunity deficiency can be persistent and can cause chronic anemia, therefore may threaten their lives. And the spectrum of disease about B19 is also wider because of the development of detection methods and research. The genome of B19 is approximately 5.6kb.The genome encodes a non-structural protein(NSl), and two structural proteins(VPl and VP2) that comprise the B19 capsid, the minor structural protein, VP1, and major structural protein, VP2. B19 capsids are composed mainly of VP2 protein, VPlprotein contributes less than 10% of capsid protein, and its specific region is probably...