Construction Of The KDR Gene Recombinant T7 Phage Vaccine And Evaluation Of Its Anti-tumor Effect

Background and Objective: Tumor growth and metastasis depend on angiogenesis, the VEGFWEGFR system plays a dominant roll in tumor angiogenesis. The breaking of immune tolerance of "self-antigens" associated with angiogenesis is an attractive approach to cancer therapy by active immunity. Vascular endothelial cell is genetic stable, without down-regulation of both MHC I and MHC II, targeting on vascular endothelial growth factor receptor is different from orthodox tumor vaccine. KDR is the major receptor in VEGF-induced tumor angiogenesis, which carries week affinity to VEGF, but strong kinase activity, and it is most important in mitogenesis, chemo-taxis and morphogenesis, which determines KDR a significant target pertaining to tumor anti-angiogenic therapy. Phage display technique is based on the linking mechanism of gene and protein. Recombinant phage vaccine, belonging to vector-viral vaccine, has advantages of both DNA vaccine and protein vaccine, which can make researchers not only modulate the stereo-configuration of protein by modifying DNA on gene level, but detect the protein displayed. It combines sequence analysis with protein function study easily and effectively. Phage T7 is virulent that can infect E-coli. The capsid protein is normally made in two forms, 10A(344aa)and 10B(397aa).Functional capsids can be composed of either 10A or 10B,or of various ratios of the proteins. 10B is produced by a translational frame-shift at amino acid(aa)...