Methylation Of Estrogen Receptor α Gene Expressing In Transitional Cell Carcinoma Of The Bladder

Objective To observe ER expression in different histological grade BTCC, assess whether aberrant methylation of ERa gene can account for loss of ER expression.Methods Expression of ER in 35 bladder cancers and 12 normal bladder tissues were respectively examined by streptavidin-peroxidase conjugated method(S—P). DNA was isolated from 23 cases of these fresh samples, which conserved in - 80°C, and then treated with sodium bisulfite for methylation-specific polymerase chain reaction. The PCR products were analyzed by 2% gel electrophoresis, contrasted with homologous normal bladder tissue. Chi-square analysis was used to determine differences in status of ER expression and ERa methylation in histological grade, clinic stage, and sex of BTCC.Results 1 , The ER expression rate was 27.03% in 35 bladder cancers, man was 29.03%, woman was 25.00%(P>0.05), there was no obvious difference in sex. In 35 BTCC, expression of ER, grade I was 83.33%, grade II was 20.83%, grade HI was 0(P<0.05), the difference has statistic significance. The level of ER expression was depressed as advance of tumors in histological grade, an inverse association was found between ER expression and histological grade. The rate of ER expression in Tis stage was 62.50% (5/8), T1 stage was 25.00% (3/12), T2 stage was 16.67%(2/12), T3 stage was 0% (0/3). In low clinic stage, ER expression rate was higher than in high...