The Clinical Research Of DNA Methylation Profiling In Urine Sediments In The Diagnosis Of Bladder Cancer

Objectives:To evaluate the diagnostic value of the assaying DNA methylation state of the 10 genes in urine sediments of bladder cancer and the controls in retrospective study.Methods:Methylation specific PCR method was used to detect the methylation profiling of 10 genes(CDH1,FANCF,LOXL1,LOXL4,pl6,SFRP1,SOX9,TIG1,TIMP3 and XAFl)which can elucidate the significance of methylation profiling in urine sediments in the diagnosis of bladder cancer.Results:The occurrence of the hypermethylated state of the genes in bladder cancer cohort(82 cases) were:CDH1 22%,FANCF 13.4%,LOXL1 40.2%,LOXL4 11%,pl6 22%,SFRP1 36.6%,SOX9 3.7%,TIG1 3.7%,TIMP3 8.5%and XAF1 70.7%.But the hypermethylated frequency of these nine genes(CDH1,FANCF,LOXL1,LOXL4,pl6,SOX9,TIG1,TIMP3 and XAF1) were also high in the noncancerous urinary lesion patients.Except for the gene SFRP1,the incidence was statistically lower(6.7%vs.36.6%,P=0.000).Therefore, inclusion of the methylated SFRP1 gene into a set often genes that was previously reported by us for methylation profiling in urine sediments has improved the sensitivity(91.5%,75/82 cases)of the bladder detection.Conclusion:MSP profiling of these 11 genes in urine sediments is a potentially robust method for the non-invasive detection of bladder cancer.