Study Of Platelet Activation In Vascular Access And Dialysis Membranes Of Hemodialysis Patients

Objective:To investigate the relationship between platelet activation and recurrent native AVF dysfunction in HD patients and evaluate its clinical significance.Methods:The studied subjects were divided into three groups (group A included 10 healthy volunteers as controls,group B included 20 HD patients with longer native AVF survival and group C included 20 HD patients with recurrent native AVF dysfunction).The expression of platelet activation markers (CD62P,CD63 and PAC-1) were measured by flow cytometry(FCM)and the results were compared among groups.Results:The level of activated platelet in group C is much higher than group A(CD62P:4.67±2.64% vs 1.31±0.88%,P<0.001;CD63:1.96±1.40% vs 0.77±0.47%,P<0.02;PAC-1:2.04±1.45% vs 0.81±0.64%,P<0.02) and group B(CD62P:2.57±1.24%,P<0.05;CD63:1.04±0.62%,P<0.02;PAC-1:0.96±0.51%,P<0.005).After matching with age,sex and non-diabetes,the percentage of activated platelet in group C is much higher than group B(age-matched subgroup:CD62P:3.89±1.46% vs 2.54±1.22%,P<0.05;PAC-1:1.49±0.85% vs 0.86±0.41%,P<0.05;male subgroup:CD62P:4.58±2.60% vs 2.58±1.13%,P<0.05;PAC-1:2.16±1.73% vs 0.90±0.38%,P<0.01;female subgroup:CD62P:4.74±2.79% vs 2.54±1.48%,P<0.05;PAC-1:1.94±1.27% vs 1.04±0.68%,P<0.01;non-diabetes subgroup:CD62P:4.01±2.40% vs 2.36±1.11%,P<0.02;PAC-1:1.82±1.45% vs 0.85±0.41%,P<0.02).In the group C,the level of activated platelet of patients with diabetes is much higher than those without diabetes(CD62P:6.20±2.72% vs...