Study Of NovaBone On Modulation Of Bone Repair

Partâ… The modulation of endochondral ossification in rat nasal chondrocyte cultures by NovaBoneObjective to explore the modulation of endochondral ossification in rat nasal chondrocyte cultures by NovaBone in vitro. Methods Chondrocytes were enzymatically isolated from the nasal septum of 21 day-old foetal Sprague Dawley rats and cultured up to 21 days in the presence of a melt-derived NovaBone and control group .the growth of the cultured chondrocyte were observed by phase contrast microscopy and MTT. A collagen-rich matrixcomposed of densely packed at the periphery of the materialchondrocyte cultures by bioactive glasses being observed by Transmission electron microscopy. The specific activity of alkaline phosphatase were determined in a by atomic absorption spectrometry . The RunX2 andCollagenX mRNA levels of chondrocyte cultured were assessed by real time RT-PCR.. Results on day 12, morphological change in contact with NovaBone granules became darker than control group corresponding to the emergence of matrixbiomineralization. Transmission electron microscopy showed a collagen-rich matrixcomposed of densely packed fibres and mineralized foci formed of needle-shaped crystals in contact with an electron-dense layer located at the periphery of the material. The specific activity of alkaline phosphatase was significant higher in NovaBone cultures on day12 and 15 than control group. Runx2 expression was higher in experiment cultures on day 12. type X collagen mRNA seemed to be up-regulated in NovaBone cultures on day 20. Conclusions this study shows NovaBone has the ability to modulate the growth of chondrocytes and endochondral ossification .