Soluble Sj 22.6/26GST Fusion Protein Is Easier To Induce The Generation Of CD4～+CD25～+ T Cells Of Mice Than Its Particulate Doseform

Research on vaccine candidates of S.japonicum showed that many antigens can not induce protection against challenge infection higher than 40%. That the single antigen only could induce limited protection or that the effective antigen has not been found was the major explanation. In fact, it was not persuasive because many experiments proved that mixture of many antigens or compound antigen could acquire quite a bit higher protectivity. However, there were still some effective vaccines of S.japonicum, such as attenuated cercariae, some antigen of S.japonicum conjugated with nanoparticles and so on. They were all not soluble antigens with Freud's adjuvant but particulate ones. In irradiated-cercariae-immunized models, much evidence suggested that Th1 subset played an important role in the mechanism of the host resistance against challenge infection. Cytokine profile of the current vaccine candidates showed that IFN-γ increased and IL-4 decreased in animal models immunized with particulate vaccines whereas IL-4 and IL-10 increased in those immunized with soluble antigens emulsified with Freud's adjuvant. In the recent years, the role of CD4~+CD25~+ regulatory T cells, the modulator of immune response, has been gradually recognized. CD4~+...