Expression Of AdipoR MRNA In Adipose Tissues Of Human And Mouse And The Effects Of TNF-α And Pioglitazone On Its Expression

Objective To observe the adiponectin receptors mRNA expression in adipose tissues of obese patients and their relations with serum leptin, TNF-α,FFA.Methods The adiponectin receptors mRNA expression level was measured by RT-PCR in adipose tissue of 19 subjects with obesity(BMI≥25 kg / m～2 ) and 29 non-obesity controls(BMI＜25 kg / m～2 ).Fasting plasma insulin(FINS), glucose(FPG),lipids, serum leptin, TNF-α , FFA were determined. Results (1)The adiponectin receptors mRNA expression was not increased in abdominal subcutaneous and omental adipose tissues when obese individuals compared with the controls(all P>0.05);Compared with abdominal subcutaneous ,the adiponectin receptors mRNA expression in omental adipose tissue was not increased in two groups(P>0.05). But the plasma TG, VLDL-C, FINS, serum leptin, TNF-α and FFA levels in obese subjects were higher than those of normal controls(P＜0.05 ～ P＜0.01). (2)Among the 48 subjects observed, the expression level of adiponectin receptors mRNA in abdominal subcutaneous adipose tissue was negatively correlated with serum leptin, FFA, FINS and H0MA-IR(P＜0.05～P＜0.01) ;and its expression level was positively correlated with ISI (P＜0.05 and <0.01 respectively). (3) In obese subjects, FFA was positively correlated with plasma TG,LDL,FINS(all P＜0.05) ;leptin was positively correlated with BMI and FINS(P＜0.01 and <0.001 respectively). Conclusions:Serum leptin, TNF-α and FFA levels in obese subjects are higher than those of nomal controls, while there is no difference in expression of adiponectin receptors mRNA in adipose tissue between two groups. Adiponectin receptors, FINS and FFA are good indexes in evaluating the insulin sensitivity.