Duplication Of The Cardiac Arrest Model In Rats By Asphyxia Method And Correlative Study Of Brain Edema In Early Stages After Cardiopulmonary Resuscitation

Part I Duplication of The Cardiac Arrest Model in Rat by Asphyxia MethodObjective: To duplicate the cardiac arrest model in rat by asphyxia method.; to investigate the effect of time of cardiac arrest(CA) on the result of resuscitation.Methods: Twenty male SD rats were divided into two groups at random:CA1min rats; CA 3min rats. Cardiac arrest was induced in rats by the intravenous injection of Suxamethonium Chloride. Cardiopulmonary resuscitation(CPR) was executed on 1min or 3min after CA,. Dynamic changes of mean arterial pressure(MAP) , heart rate (HR)and body temperature were monitored in rats by electrocardio-monitor during the stage of CPR, and the dynamic changes of blood gas were monitored by drawing blood from femoral artery. The time of ROSC, the percentage of successful resuscitation and the percentage of survival on third hour after CPR were observed.Results: 1. The change tendency of basic vital signs during CPR between two groups was nearly same , but MAP and HR in the group of CA 3min were much lower than those in the group of CA lmin before CPR(P < 0.05), nearly approach zero . Severe metabolic acidosis both existed in two groups when ROSC , indicating it was necessary to supply Sodium Bicarbonate this time.2. T he time of ROSC in the group of CA 3min was much longer than that in the group of CA 1min (P< 0.05 ), and the percentage of survival on third hour after CPR in the group of CA 3min was significantly lower than that in the group of CA lmin (P < 0.05) .Though there was no significant difference on .the percentage of ROSC between the two groups , the percentage of ROSC in the group of CA 3min showed the trend .of descent.Conclusion: 1. The duplication of the cardiac arrest model in rat by asphyxia...