Correlation Reseach Of Concentration Diversity Of Intracytoplasm Calcium Ion And Cell Apoptosis In Neuron

Objective Investigate the relationship between neuron concentrationdiversity of intracytoplasm calcium ion and cell apoptosis and analysis the mechanism how heightened neuron concentration diversity of intracytoplasm calcium ion evoke cell apoptosis.Methods To coup parietal bone of newly born Wistarn suckling rat using weight drop devise and recipe the cortex of circum-lacerating to abstract and inoculate the neuron , dyeing intracytoplasm calcium ion using Fluo-3/AM of in vitro culture neuron after inoculation 4 days and determining concentration intracytoplasm calcium ion with flow cytometre , at the same time , dyeing PtdSer using PI and Annexin V determining apoptotic index with flow cytometre, We demesh and inoculate normol neuron to be the comparision .Analysis and relationship between neuron concentration diversity of intracytoplasm calcium ion and cell apoptosis using statistics method was carry out.Results The concentration of intracytoplasm calcium ion in neuron ofcircum-lacerating is obviously higher than normal neuron and apoptotic index in neuron of circum-lacerating is also higher than normal neuron. There are difference between the high of the concentration intracytoplasm calcium ion in neuron and the increase of the apoptotic index of neuron in statistics.Conclusions The concentration of intracytoplasm calcium ion in neuron of circum-lacerating is obviously higher than normal neuron , that induce calcium ion...