Effects Of Topical Insulin Application On Neovascularization Of The Wound

Objective: In recent years, more attention has increasingly been paid to the effect of insulin and glycemia regulation on burn wounds. Studies indicate that insulin, one of the most important anabolism hormone, can promote the synthesis of the hephormone and transportation of the glucose, and increase the synthesis of protein and storage of the lipid in the adipocyte. The exogenous insulin is helpful to rectify negative nitrogen balance and promotes the formation of blood vessel. This study was to investigate the effects of topical application of small dosage of insulin on the neovascularization of the wound, the concentration of blood glucose and the angiogenesis of the wound were observed. And the mechanism of angiogenesis of the wound was also discussed so as to supply the sound theoretical basis for the topical use of insulin on the burn wound.Method: Sixty-four Wistar rats were used. The rats were subjected to 10% deeppartial thickness scalding and were randomly divided into group A (with subcutaneous injection of 0.1 U insulin into the wounds), B (with subcutaneous injection of l.OU insulin into the wounds), C (with subcutaneous injection of 0.1U insulin in the abdomen as control) and D (with subcutaneous injection of isotonic saline into the wounds as control). The injection was given every other day from the first post-scalding day until re-epithelization over the wound. Sections of wound biopsies were stained with hematoxylin-eosin staining to observe the pathological process of the wound. Blood samples were collected from the tail vein, and blood glucose concentration of each...